3. Physiology
        3.6. Haematology
            3.6.2. Transfusion medicine
                3.6.2.4. Blood compatibility test
3.6.2.4.1. Coomb's test

Coomb's test

[Ref: KB2:p186; PK1:p252-253]

Coomb's reagent

Direct Coomb's test

Direct Coomb's test is positive in

Indirect Coomb's test

Step 1: Incubation

Donor's RBCs are incubated in recipient's serum at 37C
--> Allow any IgG antibodies (from the recipient) to bind to the corresponding antigen on donor RBC
* i.e. First stage

Step 2: Washing

Removes all serum and unbound IgG in the serum
--> IgG antibodies bound to donor RBC remains

Step 3: Testing with Coomb's reagent

Coomb's reagent is added to the washed RBC

If there are IgG antibodies (from recipient's serum) bound to donor RBC
--> Anti-human-IgG IgG antibodies will bind to the IgG on two RBCs
--> Join them together
--> Agglutination
--> Positive Coomb's test

If no IgG are bound to donor RBC
--> No agglutination
--> Negative Coomb's test

Step 4: Coomb's control test

Coomb's control cells
* RBC known to be coated with IgG

Coomb's control cells are added to the negative Coomb's test
--> Will definitely cause agglutination if the reagent was active

If no agglutination when Coomb's control cells are added
--> Reagent was inactive

Indirect Coomb's test is positive when

 



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